Rabbit Anti-Human Smad3 Polyclonal: PerCP

Basic information

Name

Rabbit Anti-Human Smad3 Polyclonal: PerCP

size

100 µl

Catalog number

SPC-1285D-PCP

Price

356 €

Extended information

Total weight (kg)

1.4

Net weight (g)

0.1

Gene number

4088

Protein number

P84022

Raised in

Rabbit

Country of production

Canada

Storage recommendations

-20°C

Verified applications

WB, IHC

Stock availabilit

In Stock

Released date

6/Sep/2016

Antibody's target

Human Smad3

NCBI number

NP_005893.1

Tested reactivity

To be tested

Tested applications

To be tested

Warning information

Non-hazardous

PubMed number

Refer to PubMed

Shipping recommendations

Blue Ice or 4°C

Antibody's reactivity

Human, Mouse, Rat

Antibody in cell

Cytoplasm, Nucleus

Antibody's category

Polyclonal Antibodies

Latin name

Oryctolagus cuniculus

Antibody's purified from

Peptide Affinity Purified

Group

Polyclonals and antibodies

Conjugate

Peridinin chlorophyll (PerCP)

Antibody's full description

Rabbit Anti-Human Smad3 Polyclonal

Immunogen

A synthesized peptide derived from human Smad3

Antibody's specificity

Detects ~48kDa, endogenous levels of total Smad3

Antibody's concentration

See included datasheet or contact our support service

Tissue specificity

See included datasheet or contact our support service.

Recommended buffer for storage

PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Antibody's recommended dilutions for use

WB (1:2000), IHC (1:200); optimal dilutions for assays should be determined by the user.

Primary research fields

Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Certificate of analysis

A 1:1000 dilution of SPC-1285 was sufficient for detection of Smad3 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Antibody's other name

hMAD 3 Antibody, JV15 2 Antibody, LDS1C Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, MAD homolog 3 Antibody, Mad3 Antibody, MADH3 Antibody, SMAD 3 Antibody

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Representative figure legend

Western blot analysis of Human Hek293 cell lysates showing detection of ~58kDa Smad3 protein using Rabbit Anti-Smad3 Polyclonal Antibody (SPC-1285). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad3 Polyclonal Antibody (SPC-1285) at 1:1000. Predicted/Observed Size: ~58kDa. Rabbit Anti-Smad3 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1285)

Bibliography

1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.

Scientific context

Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.