Rabbit Anti-Human Smad2 Polyclonal: Biotin

Basic information

Name

Rabbit Anti-Human Smad2 Polyclonal: Biotin

size

100 µl

Catalog number

SPC-1284D-BI

Price

352 €

Extended information

Total weight (kg)

1.4

Net weight (g)

0.1

Gene number

4087

Country of production

Canada

Protein number

Q15796

Raised in

Rabbit

Storage recommendations

-20°C

Stock availabilit

In Stock

Released date

6/Sep/2016

NCBI number

NP_005892.1

Antibody's target

Human Smad2

Conjugation

Biotinylated

Tested reactivity

To be tested

Tested applications

To be tested

Warning information

Non-hazardous

PubMed number

Refer to PubMed

Verified applications

WB, IHC, ICC/IF

Shipping recommendations

Blue Ice or 4°C

Antibody's reactivity

Human, Mouse, Rat

Antibody in cell

Cytoplasm, Nucleus

Antibody's category

Polyclonal Antibodies

Latin name

Oryctolagus cuniculus

Antibody's purified from

Peptide Affinity Purified

Group

Polyclonals and antibodies

Antibody's full description

Rabbit Anti-Human Smad2 Polyclonal

Immunogen

A synthesized peptide derived from human Smad2

Antibody's specificity

Detects ~58kDa, endogenous levels of total Smad2

Antibody's concentration

See included datasheet or contact our support service

Recommended buffer for storage

PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide

Tissue specificity

Expressed at high levels in skeletal muscle, heart and placenta.

Test

StressMark antibodies supplies antibodies that are for research of human proteins.

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.

Antibody's recommended dilutions for use

WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.

Antibody's other name

hMAD-2 Antibody, JV18 Antibody, MAD Antibody, MAD homolog 2 Antibody, SMAD 2 Antibody, SMAD family member 2 Antibody

Primary research fields

Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Certificate of analysis

A 1:1000 dilution of SPC-1284 was sufficient for detection of Smad2 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.

Representative figure legend

Western blot analysis of Human Hek293 cell lysates showing detection of ~52kDa Smad2 protein using Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-Smad2 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1284)

Bibliography

1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.

Scientific context

Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Properties

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.