Ser423 + Ser425
Alexa conjugate 1
ALEXA FLUOR® 594
Applications with corresponding dilutions
Smad3 Ser423 + Ser425
Purified by Protein A.
Conjugated Primary Antibody
Anti-Smad3 (Ser423 + Ser425)
Alexa Fluor,ALEXA FLUOR® 594
Rabbit (Oryctolagus cuniculus)
Also known as
Smad3 (Ser423 + Ser425) Antibody
For facs or microscopy Alexa 1 conjugate.
Cross reactive species
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
These phosphorylation sites are homologous across the species listed. Smad3 (Ser423 + Ser425)
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Cross Reactive Species details
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.