Western blot: 0.5-1ug/ml
Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
0.5mg/ml if reconstituted with 0.2ml sterile DI water
This SMAD6 antibodyis to be used only for research purposes and not for diagnostics..
An amino acid sequence from the C-terminus of human SMAD6 (IKVFDFERSGLQHAPEP) was used as the immunogen for this SMAD6 antibody.
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
The stated application concentrations are suggested starting amounts. Titration of the SMAD6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
After reconstitution, the SMAD6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Mothers against decapentaplegic homolog 6 is a protein that in humans is encoded by the SMAD6 gene. It belongs to the SMAD family of proteins, which belongs to the TGFbeta superfamily of modulators. By PCR screening of a somatic cell hybrid panel and YACs, this gene was mapped to 15q22.31. This gene acts as a mediator of TGF-beta and BMP antiflammatory activity. It can suppress 1L 1R-TLR signaling through its direct interaction with PEL1 and prevent NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. SMAD6 may play a role in blocking the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding and binding to regulatory elements in target promoter regions.