Antigen affinity purified
Polyclonal (rabbit origin)
Rabbit (Oryctolagus cuniculus)
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
0.5mg/ml if reconstituted with 0.2ml sterile DI water
This SMAD5 antibodyis to be used only for research purposes and not for diagnostics..
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
An amino acid sequence from the middle region of human SMAD family member 5 (IPQIMPSISSRDVQPVAYEE) was used as the immunogen for this SMAD5 antibody (100% rat homology).
The stated application concentrations are suggested starting amounts. Titration of the SMAD5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
After reconstitution, the SMAD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Mother against decapentaplegic homolog 5, also called SMAD family member 5, is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.