SMAD5 Antibody

Basic information

Name

SMAD5 Antibody

size

0.1mg

Catalog number

R31228

Price

389 €

Details & buy    

Extended information

Recognised antigen

SMAD5

Uniprot #

Q99717

Category

Antibody

Tested applications

WB, IHC-P

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This SMAD5 antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

An amino acid sequence from the middle region of human SMAD family member 5 (IPQIMPSISSRDVQPVAYEE) was used as the immunogen for this SMAD5 antibody (100% rat homology).

Notes

The stated application concentrations are suggested starting amounts. Titration of the SMAD5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

After reconstitution, the SMAD5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

Mother against decapentaplegic homolog 5, also called SMAD family member 5, is a protein that in humans is encoded by the SAMD5 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of modulators. The gene was assigned to human chromosome 5q31. Like many other TGFbeta family members SMAD5 is involved in cell signalling and modulates signals of bone morphogenetic proteins(BMP's). It may play a role in the pathway where TGFbeta is an inhibitor of hematopoietic progenitor cells.