SMAD4 Antibody

Basic information

Name

SMAD4 Antibody

size

0.4 ml

Catalog number

F50739-0.4ML

Price

406 €

Extended information

Recognised antigen

SMAD4

Uniprot #

Q13485

Category

Antibody

Form

Purified

Purity

Purified

French translation

anticorps

Conjugation

Unconjugated

Clone

Polyclonal antibody

Tested applications

WB, IHC, IF, FACS, ELISA

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Intented use

This SMAD4 antibodyis to be used only for research purposes and not for diagnostics..

Recommended dilutions

Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Immunogen

A portion of amino acids 400-428 from the human protein was used as the immunogen for this SMAD4 antibody.

Notes

Titration of the SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

Aliquot the SMAD4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Description

In muscle physiology, SMAD4 plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). [UniProt]