SMAD4 Antibody (phospho-T277)

Basic information

Name

SMAD4 Antibody (phospho-T277)

size

0.08 ml

Catalog number

F48451-0.08ML

Price

199 €

Extended information

Uniprot #

Q13485

Category

Antibody

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Tested applications

IHC, IF, DB, ELISA

Clone

Polyclonal antibody

Recognised antigen

SMAD4 (phospho-T277)

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Recommended dilutions

Immunofluorescence: 1:10-1:50,Dot blot: 1:500,IHC (Paraffin): 1:10-1:50

Intented use

This phospho-SMAD4 antibodyis to be used only for research purposes and not for diagnostics..

Notes

Titration of the phospho-SMAD4 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Immunogen

This phospho-SMAD4 antibody was produced from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding pT277 of human SMAD4.

Storage

Aliquot the phospho-SMAD4 antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.

Description

Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily, SMAD4 is the common SMAD (co-SMAD). It promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. It may act as a tumor suppressor.