smad3 (AF9F7) Monoclonal Antibody

Basic information

Name

smad3 (AF9F7) Monoclonal Antibody

size

0.1ml

Catalog number

bsm-50352M

Price

362 €

Details & buy    

Extended information

Tested Applications

WB

Gene ID number

4088

Modification(s)

None

Target Antigen

smad3

Clone number

AF9F7

Swiss Prot

P84022

Concentration

0.5ug/ul

French translation

anticorps

Clonality

Monoclonal

Recommended dilutions

WB (1:5,000)

Conjugation

Unconjugated

Cross reactivity

Human, Mouse, Rat

Category

Primary Antibodies

Modification site(s)

Unmodified antibody

Purification method

Purified by Protein G.

Also known as

smad3 (AF9F7) Monoclonal Antibody

Long name

smad3 (AF9F7) Monoclonal Antibody

Specificity

This is a highly specific antibody against smad3.

Source

Recombinant human protein purified from E.coli (His-Smad3)

Cross reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonym names

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Background of the target antigen

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.