Smad3 (Ser423 + Ser425) Antibody

Basic information

Name

Smad3 (Ser423 + Ser425) Antibody

size

0.1ml

Catalog number

bs-3425R

Price

281 €

Details & buy    

Extended information

Gene ID number

4088

Swiss Prot

P84022

French translation

anticorps

Concentration

1ug per 1ul

Immunogen range

380-425/425

Conjugation

Unconjugated

Modification(s)

Phosphorylation

Modification site(s)

Ser423 + Ser425

Cross reactivity

Human, Mouse, Rat

Category

Primary Antibodies

Subcellular location

Cytoplasm, Nucleus

Clonality

Polyclonal antibody

Clone number

Polyclonal antibody

Tested Applications

WB, IHC-P, IF(IHC-P)

Target Antigen

Smad3 Ser423 + Ser425

Long name

Smad3 (Ser423 + Ser425) Antibody

Also known as

Smad3 (Ser423 + Ser425) Antibody

Purification method

This antibody was purified via Protein A.

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Cross reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Specificity

This is a highly specific antibody against Smad3 Ser423 + Ser425. These phosphorylation sites are homologous across the species listed.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonym names

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Background of the target antigen

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.