Smad3 (Ser425) Antibody

Basic information

Name

Smad3 (Ser425) Antibody

size

0.1ml

Catalog number

bs-5616R

Price

281 €

Details & buy    

Extended information

Gene ID number

4088

Modification site(s)

Ser425

Swiss Prot

P84022

French translation

anticorps

Immunogen range

410-425/425

Concentration

1ug per 1ul

Conjugation

Unconjugated

Target Antigen

Smad3 Ser425

Modification(s)

Phosphorylation

Cross reactivity

Human, Mouse, Rat

Category

Primary Antibodies

Subcellular location

Cytoplasm, Nucleus

Also known as

Smad3 (Ser425) PAb

Clonality

Polyclonal antibody

Clone number

Polyclonal antibody

Tested Applications

WB, IHC-P, IF(IHC-P)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser425) Primary Polyclonal Antibody

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Specificity

This is a highly specific antibody against Smad3 Ser425.

Cross reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser425 [SV(p-S)]

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonym names

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Background of the target antigen

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.