Smad2(Ser465 + Ser467) Antibody

Basic information

Name

Smad2(Ser465 + Ser467) Antibody

size

0.1ml

Catalog number

bs-3419R

Price

281 €

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Extended information

Gene ID number

4087

French translation

anticorps

Concentration

1ug per 1ul

Conjugation

Unconjugated

Modification(s)

Phosphorylation

Modification site(s)

Ser465 + Ser467

Cross reactivity

Human, Mouse, Rat

Category

Primary Antibodies

Clonality

Polyclonal antibody

Clone number

Polyclonal antibody

Tested Applications

WB, IHC-P, IF(IHC-P)

Target Antigen

Smad2 Ser465 + Ser467

Long name

Smad2(Ser465 + Ser467) Antibody

Also known as

Smad2(Ser465 + Ser467) Antibody

Purification method

This antibody was purified via Protein A.

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)

Specificity

This is a highly specific antibody against Smad2 Ser465 + Ser467.

Cross reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human SMAD2 around the phosphorylation site of Ser465/467

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonym names

Smad2p-Ser465/467; p-Smad2Ser465/467; Smad2p-S465/467; hMAD 2; hSMAD2; JV18 1; JV18; JV181; MAD; MAD Related Protein 2; MADH2; MADR2; MGC22139; MGC34440; Mothers Against Decapentaplegic Homolog 2; mothers against DPP homolog 2; SMAD 2; SMAD; SMAD2; SMAD2_HUMAN.

Background of the target antigen

Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor-regulated Smad's, activin/TGF alpha receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smad's; and the inhibitory Smad's, (Smad6 and Smad7). Smad2 consists of two highly conserved domains, the N terminal Mad homology (MH1) and the C-terminal Mad homology 2 (MH2) domains. The MH1 domain binds DNA and regulates nuclear import and transcription while the MH2 domain conserved among all the Smad's regulates Smad2 oligomerization and binding to cytoplasmic adaptors and transcription factors. Activated Smad2 associates with Smad4 and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. This translocation of Smad2 (as well as Smad3) into the nucleus is a central event in TGF beta signaling. Phosphorylation of threonine 8 in the calmodulin binding region of the MH1 domain by extracellular signal regulated kinase 1(ERK 1) enhances Smad2 transcriptional activity, which is negatively regulated by calmodulin. The regulation of Smad2 phosphorylation on threonine 8 by ERK 1 and calmodulin is critical for Smad2 mediated signaling.