Smad4 (31G4) Monoclonal Antibody

Basic information

Name

Smad4 (31G4) Monoclonal Antibody

size

0.1ml

Catalog number

bsm-50326M

Price

362 €

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Extended information

Tested Applications

IP

Gene ID number

4089

Modification(s)

None

Clone number

31G4

Target Antigen

Smad4

Cross reactivity

Human

Swiss Prot

Q13485

French translation

anticorps

Clonality

Monoclonal

Recommended dilutions

IP (2.0 ug)

Concentration

1ug per 1ul

Conjugation

Unconjugated

Category

Primary Antibodies

Modification site(s)

Unmodified antibody

Purification method

Purified by Protein G.

Long name

Smad4 (31G4) Monoclonal Antibody

Also known as

Smad4 (31G4) Monoclonal Antibody

Specificity

This is a highly specific antibody against Smad4.

Source

Recombinant human protein purified from E.coli(His-Smad4)

Cross reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Keep the antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for up to 1 year.

Properties

If you buy Antibodies supplied by Bioss Primary Unconjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonym names

JIP; DPC4; MADH4; MYHRS; Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.

Background of the target antigen

In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD4/JUN/FOS complex which forms at the AP1 promoter site; required for synergistic transcriptional activity in response to TGF-beta. May act as a tumor suppressor. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.