SMAD2/3 (Acetyl-K19) polyclonal antibody

Basic information

Name

SMAD2/3 (Acetyl-K19) polyclonal antibody

size

100ul

Catalog number

BS64092-100ul

Price

425 €

Details & buy    

Extended information

Applications

WB

BiowMW

~ 89 kDa

French translation

anticorps

Swiss-Prot

Q15796/P84022

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:500~1:1000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide derived from humanSMAD2/3 around the acetylated at Lys19.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Description

N terminal acetylation or CH3CO as epigenetic regulation of SMAD2/3 ( -K19) polyclonal antibody by NATs.

Specificity

SMAD2/3 (Acetyl-K19) polyclonal antibody detects endogenous levels of SMAD2/3 protein only when acetylated at Lys19.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage

Western blot (WB) analysis of SMAD2/3 (Acetyl-K19) polyclonal antibody at 1:500 dilution Lane1:PC12 whole cell lysate(40ug) Lane2:NIH-3T3 whole cell lysate(40ug) Lane3:Hela whole cell lysate(40ug)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; MADH2; MADR2; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; MADH3