Smad5 polyclonal antibody

Basic information

Name

Smad5 polyclonal antibody

size

100ul

Catalog number

BS5937-100ul

Price

425 €

Details & buy    

Extended information

Applications

WB

Swiss-Prot

Q99717

BiowMW

~ 52 kDa

French translation

anticorps

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:500~1:1000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Specificity

Smad5 polyclonal antibody detects endogenous levels of Smad5 protein.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide, corresponding to amino acids 250-295 of Human Smad5.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Usage

Western blot (WB) analysis of Smad5 polyclonal antibody at 1:500 dilution Lane1:A549 cell lysate Lane2:sp2/0 cell lysate Lane3:PC12 cell lysate

Alternative Name

Mothers against decapentaplegic homolog 5; MAD homolog 5; Mothers against DPP homolog 5; JV5-1; SMAD family member 5; SMAD 5; Smad5; hSmad5; SMAD5; MADH5

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.