Smad3 (phospho-S204) polyclonal antibody

Basic information

Name

Smad3 (phospho-S204) polyclonal antibody

size

100ul

Catalog number

BS4874-100ul

Price

448 €

Details & buy    

Extended information

Swiss-Prot

P84022

French translation

anticorps

BiowMW

~ 48, 55 kDa

Applications

WB IHC ICC IP

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 ICC: 1:50~1:200 IP: 1:50~1:200

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Serine 204.

Specificity

p-Smad3 (S204) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Ser204.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

Usage

Western blot (WB) analysis of p-Smad3 (S204) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate treated with UV(4h) Lane2:sp2/0 cell lysate treated with UV(4h) Lane3:H9C2 cell lysate treated with UV(4h)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.