Smad1 (phospho-S465) polyclonal antibody

Basic information

Name

Smad1 (phospho-S465) polyclonal antibody

size

100ul

Catalog number

BS4573-100ul

Price

448 €

Details & buy    

Extended information

Swiss-Prot

Q15797

Applications

WB IHC

BiowMW

~ 60 kDa

French translation

anticorps

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic phosphopeptide derived from human Smad1 around the phosphorylation site of Serine 465.

Specificity

p-Smad1 (S465) polyclonal antibody detects endogenous levels of Smad1 protein only when phosphorylated at Ser465.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name

Mothers against decapentaplegic homolog 1; MAD homolog 1; Mothers against DPP homolog 1; JV4-1; Mad-related protein 1; SMAD family member 1; SMAD 1; Smad1; hSMAD1; Transforming growth factor-beta-signaling protein 1; BSP-1; SMAD1; BSP1; MADH1; MADR1

Usage

Western blot (WB) analysis of p-Smad1 (S465) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:A549 treated with UV for 5 minutes then repair for 1 hour whole cell lysate(40ug) Lane3:A549 treated with UV for 5 minutes then repair for 3 hours whole cell lysate(40ug) Lane4:A549 treated with UV for 5 minutes then repair for 6 hours whole cell lysate(40ug) Lane5:A549 treated with UV for 5 minutes then repair for 24 hours whole cell lysate(40ug) Lane6:The Uterus tissue lysate of Mouse(40ug) Lane7:The Uterus tissue lysate of Rat(40ug)