Smad2/3 (N175) polyclonal antibody

Basic information

Name

Smad2/3 (N175) polyclonal antibody

size

100ul

Catalog number

BS3254-100ul

Price

425 €

Details & buy    

Extended information

Reactivity

Human

Swiss-Prot

P84022

Applications

WB IHC

French translation

anticorps

BiowMW

~ 55, 60 kDa

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide, corresponding to amino acids 145-190 of Human Smad3.

Specificity

Smad2/3 (N175) polyclonal antibody detects endogenous levels of Smad2/3 protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage

Western blot (WB) analysis of Smad2/3 (N175) polyclonal antibody at 1:500 dilution Lane1:MG63 whole cell lysate(40µg) Lane2:Myla2059 whole cell lysate(40µg) Lane3:HCT116 whole cell lysate(40µg) Lane4:A549 whole cell lysate(40µg)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3; Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2