Smad4 (L43) polyclonal antibody

Basic information

Name

Smad4 (L43) polyclonal antibody

size

100ul

Catalog number

BS2050-100ul

Price

425 €

Details & buy    

Extended information

Swiss-Prot

Q13485

BiowMW

~ 60 kDa

French translation

anticorps

Applications

WB IHC IF

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Immunogen

Synthetic peptide, corresponding to amino acids12-64 of Human Smad4.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Specificity

Smad4 (L43) polyclonal antibody detects endogenous levels of Smad4 protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of Smad4 (L43) pAb at 1:500 dilution Lane1:A549 whole cell lysate(40ug) Lane2:K562 whole cell lysate(40ug) Lane3:HepG2 whole cell lysate(40ug) Lane4:CT26 whole cell lysate(40ug) Lane5:H9C2 whole cell lysate(40ug)