Smad2 (P459) polyclonal antibody

Basic information

Name

Smad2 (P459) polyclonal antibody

size

100ul

Catalog number

BS1425-100ul

Price

425 €

Details & buy    

Extended information

Swiss-Prot

Q15796

BiowMW

~ 60 kDa

French translation

anticorps

Applications

WB IHC IF

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide, corresponding to amino acids 426-476 of Human Smad2.

Specificity

Smad2 (P459) polyclonal antibody detects endogenous levels of Smad2 protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

Mothers against decapentaplegic homolog 2; MAD homolog 2; Mothers against DPP homolog 2; JV18-1; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; hSMAD2; SMAD2; MADH2; MADR2

Usage

Western blot (WB) analysis of SMAD2 pAb at 1:500 dilution Lane1:HCC827 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:The Heart tissue lysate of Mouse(40ug) Lane4:The Heart tissue lysate of Rat(20ug)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.