Smad3 (phospho-T179) polyclonal antibody

Basic information

Name

Smad3 (phospho-T179) polyclonal antibody

size

100ul

Catalog number

AP0327-100ul

Price

448 €

Details & buy    

Extended information

Applications

WB

Swiss-Prot

P84022

French translation

anticorps

BiowMW

~ 48, 55 kDa

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:1000~1:2000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

Synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Threonine 179.

Specificity

p-Smad3 (T179) polyclonal antibody detects endogenous levels of Smad3 protein only when phosphorylated at Thr179.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of p-Smad3 (T179) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with TNFa(20ng/ml,15mins) Lane2:Raw264.7 cell lysate treated with TNFa(20ng/ml,15mins) Lane3:PC12 cell lysate treated with TNFa(20ng/ml,15mins)