Smad3 (phospho-S423/S425) Rabbit monoclonal antibody

Basic information

Name

Smad3 (phospho-S423/S425) Rabbit monoclonal antibody

size

50ul

Catalog number

BS9938M-50ul

Price

408 €

Extended information

Swiss-Prot

P84022

BiowMW

~ 55 kDa

French translation

anticorps

Reactivity

Human, Mouse

Applications

WB ICC/IF IHC

Latin name

Oryctolagus cuniculus

Immunogen

Recombinant antibody.

Purification&Purity

Protein A affinity purified

Application_all

WB: 1:1000 ICC/IF: 1:50-1:200 IHC: 1:50-1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity

This antibody detects endogenous levels of Smad3 only when phosphorylated at Ser423 and/or Ser425.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Usage

Western blot (WB) analysis of Smad3 (phospho-S423/S425) Rabbit mAb at 1:1000 dilution Lane1:A431 whole cell lysate Lane2:A431 whole cell lysate treated with TGF β

Alternative Name

Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3; SMAD3; MADH3

About

Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.Rabbits are used for polyclonal antibody production by Bioworld. Rabbit antibodies are very stable and can be stored for several days at room temperature. Bioworld adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.