Smad3 (Ser423) Antibody, Cy7 Conjugated

Basic information

Name

Smad3 (Ser423) Antibody, Cy7 Conjugated

size

0.1ml

Catalog number

bs-2225R-Cy7

Price

368 €

Details & buy    

Extended information

Additional conjugation

Cy7

Conjugation

Cy7

Gene ID number

4088

Modification site

Ser423

French translation

anticorps

Tested applications

IF(IHC-P)

Clonality

Polyclonal

Concentration

1ug per 1ul

Excitation Emission

743nm/767nm

Target Antigen

Smad3 Ser423

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser423) Polyclonal Antibody, Cy7 Conjugated

Specificity

This is a highly specific antibody against Smad3 Ser423.

Conjugated

These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

Smad3 phospho Ser423; Smad3 phospho S423; p-Smad3 Ser423; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

Antigen background

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.