Smad3 (Ser423 + Ser425) Antibody, HRP Conjugated

Basic information

Name

Smad3 (Ser423 + Ser425) Antibody, HRP Conjugated

size

0.1ml

Catalog number

bs-3425R-HRP

Price

368 €

Extended information

Conjugation

HRP

Excitation Emission

N/A

Gene ID number

4088

Swiss Prot

P84022

Tested applications

WB, IHC-P

French translation

anticorps

Clonality

Polyclonal

Immunogen_range

380-425/425

Concentration

1ug per 1ul

Additional conjugation

HRP Conjugated

Modification

Phosphorylation

Modification site

Ser423 + Ser425

Crossreactivity

Human, Mouse, Rat

Subcellular location

Cytoplasm, Nucleus

Clone

Polyclonal antibody

Target Antigen

Smad3 Ser423 + Ser425

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Purification method

This antibody was purified via Protein A.

Long name

Smad3 (Ser423 + Ser425) Antibody, HRP Conjugated

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Specificity

This is a highly specific antibody against Smad3 Ser423 + Ser425. These phosphorylation sites are homologous across the species listed.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Synonyms

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Antigen background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.