anti-SMAD3 Antibody

Basic information

Name

anti-SMAD3 Antibody

size

0.05 mg

Catalog number

49-315

Price

180 €

Details & buy    

Extended information

NCBI GENE nr

4088

GENE symbol

SMAD3

Protein nr

P84022

Ncbi nr

P84022

Ab raised in

Rabbit

Omim nr

51338669

French translation

anticorps

Type

Polyclonal

Vial with antibody

in solution

Source

Homo sapiens

Antibodie's label

unlabelled serum

Purity

Protein G Column

Antigene name

SMAD family member 3

Immuno application

Western Blot, immuno-histo-chemistry

Suspention, pH, azide

PBS, 0.2% gelatin, 0.05% sodium azide.

Works with

Bovine, Dog, Human, Macaque, Monkey, Mouse, Pig, Rat

Antigen

SMAD3 antibody was made against a portion of amino acids 100 - 150 of SMAD3 (Human).

Antibody advice

SMAD3 antibody can be used in ELISA, Western Blot, and immunohistochemistry starting at 5 ug/mL.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Keep at

SMAD3 antibody can be stored short term 4 grades C. For long term storage aliquot and store at -20 grades C. As with all antibodies avoid freeze/thaw cycles.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Gene names

SMAD3, HSPC193, HMAD-3, HsT17436, LDS1C, LDS3, Mad homolog JV15-2, MAD3, MADH3, Mothers against DPP homolog 3, JV15-2, SMA- and MAD-related protein 3, SMAD family member 3, MAD homolog 3, HSMAD3, Mad protein homolog, SMAD 3

Gene info

SMAD3, a member of SMAD family of proteins, is involved in TGF-β signaling cascade. A human homolog of Drosophila Mad protein, SMAD3 consists of N-terminal DNA-binding MH1 domain, a linker region and a C-terminal MH2 domain.It is a receptor regulated SMAD (R-SMAD) that functions downstream of TGF-β and activin receptors and mediates their signaling. It is recruited by SARA (Smad anchor for receptor activation) to the receptor kinase for phosphorylation. Upon phosphorylation, SMAD3 disassociates from SARA, forms a complex with SMAD4 and transmigrates into the nucleus where it complexes with other cofactors and acts as a transcription factor. It has an indispensable role in cell proliferation, differentiation, apoptosis and formation of ECM. Loss of SMAD3 results in Childhood T-cell leukemia. Smad 3 (Mothers against decapentaplegic homolog 3), a transcriptional regulator, is activated by TGF-β and Activin 1 receptor kinase. SMAD3 recognizes an 8 bp palindromic sequence GTCTAGAC. Upon phosphorylation at two extreme C-terminal serines, SMAD3 is translocated into the nucleus where is forms complexes other SMAD proteins, depending on the activation pathway, and regulates the transcription of target genes.