Vial with antibody
ELISA, Western Blot
SMAD family member 6
SMAD6, HsT17432, MADH6, MADH7, AOVD2
Antibody is purified by peptide affinity chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD6.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD6 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
SMAD6 antibody can be used for detection of SMAD6 by ELISA at 1:312500. SMAD6 antibody can be used for detection of SMAD6 by western blot at 1 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
SMAD6 (mothers against decapentaplegic homolog 6) is an antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit selectively BMP (bone morphogenetic proteins) signaling by competing with the co-SMAD SMAD4 for receptor-activated SMAD1. SMAD6 is an inhibitory SMAD (I-SMAD) or antagonistic SMAD.