Vial with antibody
SMAD family member 3
Human, Mouse, Rat, Dog, Zebrafish
ELISA, Western Blot, immuno-histo-chemistry
SMAD3, LDS3, LDS1C, MADH3, JV15-2, HSPC193, HsT17436
Antibody is purified by protein A chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD3.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD3 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
SMAD3 is a member of Smad family proteins. SMAD family is known to be important cytoplasmic mediators of signals from the transforming growth factor-beta (TGF-beta) receptor serine/threonine kinases.
SMAD3 antibody can be used for detection of SMAD3 by ELISA at 1:62500. SMAD3 antibody can be used for detection of SMAD3 by western blot at 2.0 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.