Vial with antibody
ELISA, Western Blot
SMAD family member 7
SMAD7, CRCS3, MADH7, MADH8
Antibody is purified by peptide affinity chromatography method.
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human SMAD7.
Suspention, pH, azide
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store SMAD7 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
SMAD7 antibody can be used for detection of SMAD7 by ELISA at 1:12500. SMAD7 antibody can be used for detection of SMAD7 by western blot at 1.0 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Smad7 acts to functionally inactivate RB and de-repress E2F without blocking the activation of TbetaRI and the nuclear translocation of Smad2/3, allowing TGF-beta1 to exert effects in a cancer cell that is resistant to TGF-beta1-mediated growth inhibition.