anti-SMURF2 Antibody

Basic information

Name

anti-SMURF2 Antibody

size

0.02 mg, 0.1 mg

Catalog number

7773

Price

180 €

Details & buy    

Extended information

Immunoglobulin

IgG

NCBI GENE nr

64750

Ab raised in

Rabbit

Protein nr

Q9HAU4

GENE symbol

SMURF2

Quantity per ml

1 mg/mL

Omim nr

12232397

French translation

anticorps

Ncbi nr

NP_073576

Type

Polyclonal

Vial with antibody

in solution

Source

homo sapiens

Antibodie's label

unlabelled serum

Works with

Human, Mouse, Rat

KDa

Predicted: 82 kDa Observed: 85 kDa

Antigene name

SMAD specific E3 ubiquitin protein ligase 2

Immuno application

ELISA, Western Blot, immuno-histo-chemistry, immuno fluorescence

Suspention, pH, azide

SMURF2 Antibody is supplied in PBS containing 0.02% sodium azide.

Purity

SMURF2 antibody is affinity chromatography purified via peptide column.

Antigen

SMURF2 antibody was made against a 17 amino acid peptide near the amino terminus of human SMURF2.

Spicific to

SMURF2 antibody is human, mouse and rat reactive. SMURF2 is predicted to not cross-react with SMURF1.

Gene names

SMURF2 Antibody: E3 ubiquitin-protein ligase SMURF2, SMAD ubiquitination regulatory factor 2, hSMURF2

Keep at

SMURF2 antibody can be stored at 4 grades C for three months and -20 grades C, stable for up to one year.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antibody advice

SMURF2 antibody can be used for detection of SMURF2 by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL.

Gene info

SMURF2 (SMAD ubiquitin regulatory factor 2) is a negative regulator of TGF-beta signaling (1). SMURF1 and SMURF2 are members of HECT domain E3 ubiquitin ligase which are involved in the enzymatic reactions of the Ub conjugating pathway (1,2). SMURF2 is widely expressed and was initially identified as an inhibitor of TGF-beta/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation (3). Studies have shown that SMURF2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20 (3). SMURF2 associates constitutively with SMAD7 (4).