Concentration of antibody:
Target of this antibody:
Clonality of antibody:
This antibody reacts with:
Synthetic peptide of human MAD1
This antibody has been succesfully tested for use in following methods:
Western blot, Immunoprecipitation
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody:
100 µl affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 1% BSA and 0.02% thimerosal.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Alternative names of antibody target:
MAD1, MAD-1, MADL1, HsMAD1, Mitotic arrest deficient-like 1, PIG9, p53 inducible protein 9, TP53I9, tumor protein p53 inducible protein 9, TXBP181, Tax binding protein-181
Western blotting (1:500 – 1:2000) and Immunoprecipitation. HeLa cell lysate can be used as a positive control. However, the optimal concentrations should be determined individually. The antibody recognizes the MAD1 of human and mouse origins. Reactivity to other species has not been tested.
Cell cycle progression is subject to arrest at the mitotic spindle assembly checkpoint in response to incorrect spindle fiber assembly. MAD1 and MAD2 (for mitotic arrest-deficient 1 and 2) are components of the mitotic spindle checkpoint. Incorrect spindle assembly in normal cells leads to mitotic arrest. MAD1 prevents the onset of anaphase until all chromosomes are aligned correctly at the metaphase plate and is crucial for anchoring MAD2L1 to the nuclear periphery. It also plays an important role in septum positioning. MAD1 can form a homo-dimer, but may also form a heterodimer with MAD2 to form the tetrameric MAD1L1-MAD2L1 core complex. MAD1 localizes primarily to the nucleus, but during mitosis, it moves from a nuclear distribution to the centrosome, to the spindle midzone and then on to the midbody. MAD1 activity is induced by BUB1 and the protein is hyperphosphorylated after mitotic spindle damage and/or in late S through M phase. Defects in the gene encoding for MAD1, MAD1L1, play a major role in the development and progression of various cancer types.