Smad3 Antibody (pSer425): FITC

Basic information

Name

Smad3 Antibody (pSer425): FITC

size

100 µl

Catalog number

SPC-1427D-FITC

Price

484 €

Details & buy    

Extended information

Net Weight (g)

0.1

Gross Weight (kg)

1.4

Gene ID

4088

Conjugate

FITC

Immunogen Species

Human

Storage Temperature

-20ºC

Swiss-Prot

P84022

Host Species

Rabbit

Applications

WB, IHC

French translation

anticorps

Category

Antibodies

Product Type

Polyclonal

Accession Number

NP_001138574.1

Target

Smad3 (pSer425)

Shipping Temperature

Blue Ice or 4ºC

Species Reactivity

Human, Mouse, Rat

Conjugation

Anti-FITC Antibody

Purification

Peptide Affinity Purified

Field of Use

For in vitro research use only.

Storage Buffer

PBS, pH 7.4, 150mM NaCl, 50% glycerol, 0.02% sodium azide

Immunogen

Synthetic phospho-peptide from human Smad3 around the phosphorylation site of serine 425 (pSer425).

Antibody Dilution

WB (1:250-1:1000); IHC (1:25-1:100); optimal dilutions for assays should be determined by the user.

Specificity

Detects endogenous levels of Smad3 only when phosphorylated at serine 425 (pSer425). Detects a band at ~58 kDa.

Cellular Localization

Nucleus, Cytoplasm, Cytosol, Nuclear Chromatin, Nuclear Inner Membrane, Nucleoplasm, Plasma Membrane, Receptor Complex

Research Area(s)

Cell Signaling, Epigenetics and Nuclear Signaling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cancer, Cancer Metabolism, Response to Hypoxia, Cell Signaling, Metabolism, Metabolism processes, Hypoxia

Alternative Name(s)

Smad3 Antibody, Mothers against decapentaplegic homolog 3 Antibody, Mad3 Antibody, SMAD3_HUMAN Antibody, DKFZp686J10186 Antibody, SMAD Antibody, HSPC193 Antibody, SMAD family member 3 Antibody, hMAD-3 Antibody, MGC60396 Antibody, MAD homolog 3 Antibody, HST17436 Antibody, JV15-2 Antibody, MAD (mothers against decapentaplegic Drosophila) homolog 3 Antibody, JV152 Antibody, Mad homolog JV15 2 Antibody, SMAD 3 Antibody, JV15 2 Antibody, DKFZP586N0721 Antibody, MADH3 Antibody, MAD, Mothers against DPP homolog 3 Antibody, hSMAD3 Antibody, MADH 3 Antibody, LDS3 Antibody, Mad protein homolog Antibody, SMA and MAD related protein 3 Antibody, LDS1C Antibody, hMAD 3 Antibody

Properties

If you buy Antibodies supplied by StressMarq they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This StressMarq Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMarq FITC is highly sensitive to pH extremes.