Rat Smad2(Mothers Against Decapentaplegic Homolog 2) ELISA Kit

Basic information

Name

Rat Smad2(Mothers Against Decapentaplegic Homolog 2) ELISA Kit

size

96T

Catalog number

ELK7676

Price

608 €

Details & buy    

Extended information

Assay length

3h

Standard

10ng/mL

Assay Type

Sandwich

Sensitivity

0.058ng/mL

Detection range

0.156-10ng/mL

Latin name

Rattus norvegicus

Research Area

Signal transduction;Apoptosis;Tumor immunity;

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Alternative Names

JV18; JV18-1; MADH2; MADR2; HMAD-2; HSMAD2; Mad-related protein 2

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mothers Against Decapentaplegic Homolog 2 (Smad2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mothers Against Decapentaplegic Homolog 2 (Smad2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mothers Against Decapentaplegic Homolog 2 (Smad2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.