Recombinant Mouse Smad5 Protein 100µg

Basic information

Name

Recombinant Mouse Smad5 Protein 100µg

size

100µg

Catalog number

PKSM040515-100µg

Price

716 €

Details & buy    

Extended information

Activity

NA

Fusion tag

NA

Accession

P97454

AP Mol Mass

57 kDa

Mol Mass

52.2 kDa

Sequence

Thr2-Ser465

Group

recombinants

Latin name

Mus musculus

Expressed Host

Baculovirus-Insect Cells

Source

Recombinants or rec. proteins

Purity

> 90 % as determined by SDS-PAGE

Synonym

1110051M15Rik;AI451355;Dwf-C;Madh5;MusMLP

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Formulation

Lyophilized from sterile 20mM Tris, 500mM Nacl,10% glycerol, pH8.0

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background

SMAD5 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD5 is involved in the TGF-beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. It is also involved in cell signalling and modulates signals of bone morphogenetic proteins (BMP's). The binding of ligands causes the oligomerization and phosphorylation of the SMAD5 protein. SMAD5 is a receptor regulated SMAD (R-SMAD) and is activated by bone morphogenetic protein type 1 receptor kinase.