Recombinant Mouse Smad2 Protein (His & GST tag)

Basic information

Name

Recombinant Mouse Smad2 Protein (His & GST tag)

size

20µg

Catalog number

PKSM040517-20µg

Price

356 €

Details & buy    

Extended information

Activity

NA

AP Mol Mass

90 kDa

Mol Mass

80 kDa

Conjugation

histidine

Sequence

Ser2-Ser467

Fusion tag

N-His & GST

Group

recombinants

Latin name

Mus musculus

Accession

Q62432-Isoform Long

Expressed Host

Baculovirus-Insect Cells

Source

Recombinants or rec. proteins

Purity

> 90 % as determined by SDS-PAGE

Synonym

7120426M23Rik;Madh2;Madr2;mMad2;Smad-2

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Formulation

Lyophilized from sterile 20mM Tris, 500mM Nacl, pH 8.0, 10% glycerol, 3mM DTT

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background

SMAD2 is a member of the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD2 mediates the signal of the TGF-beta, and therefore regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. SMAD2 is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. SMAD2 is the downstream signal transducers of TGF-beta-1 in human dental pulp cells. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. Phosphorylated SMAD2 is able to form a complex with SMAD4 or SARA. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression.