Recombinant Mouse Smad3 Protein (His & GST tag)

Basic information

Name

Recombinant Mouse Smad3 Protein (His & GST tag)

size

20µg

Catalog number

PKSM040402-20µg

Price

239 €

Details & buy    

Extended information

Activity

NA

Accession

P84025

AP Mol Mass

75.9 kDa

Mol Mass

75.9 kDa

Conjugation

histidine

Sequence

Met1-Ser425

Fusion tag

N-His & GST

Latin name

Mus musculus

Group

recombinants

Synonym

AU022421;Madh3

Expressed Host

Baculovirus-Insect Cells

Source

Recombinants or rec. proteins

Purity

> 85 % as determined by SDS-PAGE

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method

Formulation

Lyophilized from sterile 20mM Tris, 500mM NaCl, 2mM GSH, 10% glycerol, pH 7.4

Stability and Storage

Samples are stable for up to twelve months from date of receipt at -70℃.Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Shipping

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Test

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Background

SMAD3 belongs to the SMAD family. Members of this family mediate signal transduction by the TGF-beta/activin/BMP-2/4 cytokine superfamily from receptor Ser/Thr protein kinases at the cell surface to the nucleus. SMAD3 is involved in cell signalling. It modulates signals of activin and TGFβ's. Binding of SMAD3 with SMAD4 enables its transmigration into the nucleus where it forms complexes with other proteins and acts as a transcription factor. SMAD3 is a receptor-regulated SMAD (R-SMAD). In mice, mutation of SMAD3 has been linked to colorectal adenocarcinoma, increased systemic inflammation, and accelerated wound healing. Increased SMAD3 activity has been implicated in the pathogenesis of scleroderma. Smad3 is also a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes.