Recombinant Human SMAD Family Member 4/SMAD4/DPC4 Protein(C-6His)

Basic information

Name

Recombinant Human SMAD Family Member 4/SMAD4/DPC4 Protein(C-6His)

size

10µg

Catalog number

PKSH033066-10µg

Price

149 €

Details & buy    

Extended information

Activity

NA

Fusion tag

C-6His

Accession

Q13485

AP Mol Mass

62 kDa

Expressed Host

E.coli

Mol Mass

61.5 kDa

Sequence

Met1-Asp552

Group

recombinants

Source

Recombinants or rec. proteins

Purity

＞85% as determined by reducing SDS-PAGE.

Endotoxin

＜1.0 EU per µg as determined by LAL test.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl，pH8.0.

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Synonym

Mothers Against Decapentaplegic Homolog 4; MAD Homolog 4; Mothers Against DPP Homolog 4; Deletion Target in Pancreatic Carcinoma 4; SMAD Family Member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4; MADH4

Stability and Storage

Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

SMAD Family Member 4 (SMAD4) is a cytoplasmic protein that belongs to the Dwarfin/SMAD family. SMAD4 contains one MH1 (MAD homology 1) domain and one MH2 (MAD homology 2) domain. It is the component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. SMAD4 promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. SMAD4 may act as a tumor suppressor. It positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. Mutations or deletions in SMAD4 have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.