Recombinant Human Mothers Against Decapentaplegic Homolog 2/SMAD2 Protein(N-6His-Flag)
10µg
PKSH032762-10µg
203 €
NA
Flag
60 kDa
E.coli
Q15796
54.6 kDa
Ser2-Ser467
N-6His-Flag
recombinants
Recombinants or rec. proteins
＞90% as determined by reducing SDS-PAGE.
＜1.0 EU per µg as determined by LAL test.
Lyophilized from a 0.2 μm filtered solution of 20mM PB,500mM NaCl, pH7.5.
The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Mothers against decapentaplegic homolog 2; MAD homolog 2; Mad-related protein 2; hMAD-2; SMAD family member 2; SMAD 2; Smad2; Hsmad2; SMAD2; MADH2; MADR2
Lyophilized protein should be stored at < -20℃, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7℃ for 2-7 days.Aliquots of reconstituted samples are stable at < -20℃ for 3 months
Mothers against decapentaplegic homolog 2(SMAD2) is a cytoplasm protein which belongs to the dwarfin/SMAD family. Smad proteins undergo rapid nuclear translocation upon stimulation by transforming growth factor and in so doing transduce the signal into the nucleus. Receptor-regulated SMAD is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. SMAD2 is contains 1 MH1 (MAD homology 1) domain and1 MH2 (MAD homology 2) domain. It is expressed at high levels in skeletal muscle, endothelial cells, heart and placenta. It binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. It may act as a tumor suppressor in colorectal carcinoma. And SMAD2 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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