Rabbit Anti Human SMAD5 Antibody-FITC

Basic information

Name

Rabbit Anti Human SMAD5 Antibody-FITC

size

100ug

Catalog number

CSB-PA859108LC01HU_100ug

Price

302 €

Extended information

Antibody isotype

IgG

Conjugation

FITC

Purity

>95%

Species reactivity

Human

Tested applications & recommended dilutions

ELISA

Immunogen species

Human

UniProt ID

Q99717

French translation

anticorps

Additional conjugation

Anti-FITC Antibody

Purification method

Protein G purified

Clonality

Polyclonal antibody

Synonym name

SMAD5 Human Antibody

Latin name

Oryctolagus cuniculus

Antibody category

Polyclonal Antibodies

Format

Liquid, diluted in buffer

Long name

Rabbit Antibody against Human SMAD5, FITC

Short name of the antigen

Mothers against decapentaplegic homolog 5

Immunogen type

Recombinant human Mothers against decapentaplegic homolog 5 protein (1-209AA)

Buffer composition

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Background

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

Alias

Mothers against decapentaplegic homolog 5MAD homolog 5Mothers against DPP homolog 5,JV5-1,SMAD family member 5,SMAD 5,Smad5,hSmad5,SMAD5,MADH5

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Shipping and storage

SMAD5 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.

About

Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Properties

If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Cusabio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Cusabio FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.