Rabbit Anti Human SMAD3 Antibody-Biotin

Basic information

Name

Rabbit Anti Human SMAD3 Antibody-Biotin

size

50ug

Catalog number

CSB-PA021788LD01HU_50ug

Price

212 €

Extended information

Antibody isotype

IgG

Purity

>95%

Species reactivity

Human

Tested applications & recommended dilutions

ELISA

Immunogen species

Human

Conjugation

Biotin

UniProt ID

P84022

French translation

anticorps

Additional conjugation

Biotinylated

Purification method

Protein G purified

Clonality

Polyclonal antibody

Synonym name

SMAD3 Human Antibody

Latin name

Oryctolagus cuniculus

Antibody category

Polyclonal Antibodies

Format

Liquid, diluted in buffer

Alias

JV15-2 SMAD family member 3 SMAD3 MADH3

Long name

Rabbit Antibody against Human SMAD3, Biotin

Immunogen type

Recombinant human Mothers against decapentaplegic homolog 3 protein (1-425AA)

Buffer composition

Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.

Shipping and storage

SMAD3 Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.

About

Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Properties

If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.