Area of research
DNA & RNA
Polyclonal Antibodies, Purified
Human: Thr179, Mouse: Thr179, Rat: Thr179
WB: 1:500-1:1000, IHC: 1:50-1:100, ELISA: 1:1000
Aliquot and store at -20 deg C. Avoid repeated freeze thaw cycles
Smad3 antibody detects endogenous levels of Smad3 only when phosphorylated at threonine 179
Form & Buffer
Provided in PBS buffer (without Mg2+and Ca2+), pH 7.4, 150 mM NaCl with 0.02% NaN3 and 50% glycerol
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Type of Immunogen
Smad3 antibodies were raised in Rabbit using synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of threonine 179 (P-E-TP-P-P) .