Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit

Basic information

Name

Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit

size

96 Tests

Catalog number

EKE60599

Price

759 €

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Extended information

Stability

NA

Precision

NA

Specificity

NA

Target Name

NA

Species Reactivity

Human

Assay Type

Sandwich

Shipping Conditions

Ice packs

Assay Time

4.5 hours

Sensitivity

0.19 ng/mL

Target's alterntive name

Smad4/MADH4

Detection Method

Colorimetric

Detection Range

0.31-20 ng/mL

Estimated Turnaround Time

6-11 business days

Sample Type

Serum, Plasma, Biological Fluids

Storage Temperature

Short term: 4°C; Long term: see manual.

Application

For research use only. Not for diagnostic procedures.

Quality Systems

The kit is manufactured at ISO 9001 certified facilities.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Cross Activity

No significant cross-reactivity or interference was observed.

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Shelf life

Use Human Smad4/MADH4 (Mothers Against Decapentaplegic Homolog 4) ELISA Kit before 6 months

Research Area

Cancer, Cardiovascular, Epigenetics and Nuclear Signaling, Metabolism, Signal transduction, Stem cells

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Test Principle

This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Smad4/MADH4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Smad4/MADH4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Smad4/MADH4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human Smad4/MADH4. The concentration of Human Smad4/MADH4 in samples can be calculated by comparing the OD of the samples to the standard curve.