Rat Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) ELISA Kit

Basic information

Name

Rat Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) ELISA Kit

size

96 Tests

Catalog number

EKU09297

Price

1028 €

Extended information

Assay Time

3 hours

Shipping Conditions

Ice packs

Sensitivity

0.27 ng/mL

Detection Method

Colorimetric

Detection Range

0.781-50 ng/mL

Research Area

Enzyme & Kinase

Latin name

Rattus norvegicus

Estimated Turnaround Time

6-11 Business days

Species Reactivity

Rattus norvegicus (Rat)

Assay Type

Double-antibody Sandwich

Storage Temperature

Short term: 4°C; Long term: see manual.

Target Name

Smad Specific E3 Ubiquitin Protein Ligase 2

Application

For research use only. Not for diagnostic procedures.

Sample Type

Tissue homogenates, Cell lysates and other biological fluids

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Target's alterntive name

SMAD ubiquitination regulatory factor 2, E3 ubiquitin-protein ligase SMURF2

Shelf life

Use Rat Smad Specific E3 Ubiquitin Protein Ligase 2 (SMURF2) ELISA Kit before 8 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Specificity

This assay has high sensitivity and excellent specificity for detection of Smad Specific E3 Ubiquitin Protein Ligase 2

Test Principle

No significant cross-reactivity or interference between Smad Specific E3 Ubiquitin Protein Ligase 2 and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Smad Specific E3 Ubiquitin Protein Ligase 2 and analogues was observed.

About

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Smad Specific E3 Ubiquitin Protein Ligase 2 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Smad Specific E3 Ubiquitin Protein Ligase 2 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.