Multi-species Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit

Basic information

Name

Multi-species Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit

size

96 Tests

Catalog number

EKU09013

Price

830 €

Details & buy    

Extended information

Assay Time

3 hours

Shipping Conditions

Ice packs

Sensitivity

0.132 ng/mL

Detection Method

Colorimetric

Detection Range

0.312-20 ng/mL

Estimated Turnaround Time

6-11 Business days

Research Area

Developmental science

Assay Type

Double-antibody Sandwich

Storage Temperature

Short term: 4°C; Long term: see manual.

Target Name

Mothers Against Decapentaplegic Homolog 1

Application

For research use only. Not for diagnostic procedures.

Test

ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED

Precaution of Use

The Stop Solution is acidic. Do not allow to contact skin or eyes.

Species Reactivity

Homo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat)

Quality Systems

The kit is manufactured at ISO 9001 and ISO 13485 certified facilities.

Sample Type

Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids

Shelf life

Use Multi-species Mothers Against Decapentaplegic Homolog 1 (Smad1) ELISA Kit before 8 months

Properties

E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays

Specificity

This assay has high sensitivity and excellent specificity for detection of Mothers Against Decapentaplegic Homolog 1

Test Principle

No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 and analogues was observed.

Cross Activity

No significant cross-reactivity or interference between Mothers Against Decapentaplegic Homolog 1 and analogues was observed.

Target's alterntive name

BSP1, JV4-1, JV41, MADH1, MADR1, SMAD Family Member 1, Mad-related protein 1, Transforming growth factor-beta-signaling protein 1, Mothers against DPP homolog 1

Description

Monoclonals are usually produced in mouse as host species and polyclonals in rabbit or goat. Multi species reactivity is achieved by selecting consensus epitopes when blasting the gene for a reactive, innugenic epitope that is the same in the different target species.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mothers Against Decapentaplegic Homolog 1 were tested on 3 different plates, 8 replicates in each plate.CV (%) = SD/meanX100;Intra-Assay: CV

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.