Country of production
To be tested
To be tested
Refer to PubMed
Blue Ice or 4°C
Human, Mouse, Rat
Antibody in cell
Antibody's purified from
Peptide Affinity Purified
Polyclonals and antibodies
Antibody's full description
Rabbit Anti-Human Smad4 Polyclonal
See included datasheet or contact our support service
See included datasheet or contact our support service.
Recommended buffer for storage
PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide
Detects ~60kDa, endogenous levels of total Smad4 protein.
Synthesized peptide derived from internal of human Smad4.
StressMark antibodies supplies antibodies that are for research of human proteins.
Contact our support service to receive datasheet or other technical documentation.
Antibody's recommended dilutions for use
WB (1:1000), ELISA (1:10000); optimal dilutions for assays should be determined by the user.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Certificate of analysis
A 1:1000 dilution of SPC-1286 was sufficient for detection of Smad4 in 10 µg of HeLa, Cos7, HepG2, HUVEC cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.
Primary research fields
Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic, Cardiovascular System, Heart, Cardiogenesis, Cell Signaling, Metabolism, Metabolism processes, Hypoxia
1. Heldin C.H. et al. (1997) Nature. 390: 465-71. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-94. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-91. 4. Whitman M. (1998) Genes Dev. 12: 2445-62. 5. Wu G. et al. (2000) Science. 287: 92-7.
Antibody's other name
(Small) Mothers Against Decapentaplegic Antibody, Deleted in Pancreatic Carcinoma 4 Antibody, DPC 4 Antibody, hSMAD4 Antibody, JIP Antibody, MADH 4 Antibody, Mothers against decapentaplegic homolog 4 Antibody, SMAD 4 Antibody, SMAD mothers against DPP homolog 4 Antibody
Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Smad4 mediates all activities through interaction with various other Smad family members.
Representative figure legend
Western blot analysis of Human, Monkey HeLa, Cos7, HepG2, HUVEC cell lysates showing detection of ~60kDa Smad4 protein using Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286). Lane 1: Human HeLa. Lane 2: Monkey Cos7. Lane 3: Human HepG2. Lane 4: Human HUVEC. Lane 5: Treated with the immunizing peptide. Primary Antibody: Rabbit Anti-Smad4 Polyclonal Antibody (SPC-1286) at 1:1000. Predicted/Observed Size: ~60kDa. Rabbit Anti-Smad4 Antibody used in Western blot (WB) on HeLa, Cos7, HepG2, HUVEC cell lysates (SPC-1286)
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
This StressMark antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. StressMark antibodies FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.