Rabbit Anti-Human Smad2 Polyclonal: ATTO 680

Basic information


Rabbit Anti-Human Smad2 Polyclonal: ATTO 680


100 µl

Catalog number



357 €

Extended information

Total weight (kg)


Net weight (g)


Gene number


Raised in


Storage recommendations


Protein number


Country of production


Stock availabilit

In Stock

Released date


NCBI number


Antibody's target

Human Smad2

Tested reactivity

To be tested

Tested applications

To be tested

Warning information


Verified applications


PubMed number

Refer to PubMed

Shipping recommendations

Blue Ice or 4°C

Antibody's reactivity

Human, Mouse, Rat

Antibody in cell

Cytoplasm, Nucleus

Antibody's category

Polyclonal Antibodies

Latin name

Oryctolagus cuniculus

Antibody's purified from

Peptide Affinity Purified


Polyclonals and antibodies

Antibody's full description

Rabbit Anti-Human Smad2 Polyclonal


A synthesized peptide derived from human Smad2

Antibody's specificity

Detects ~58kDa, endogenous levels of total Smad2

Antibody's concentration

See included datasheet or contact our support service

Recommended buffer for storage

PBS pH 7.4, 50% glycerol, 150mM NaCl, 0.02% sodium azide

Tissue specificity

Expressed at high levels in skeletal muscle, heart and placenta.

Antibody's datasheet

Contact our support service to receive datasheet or other technical documentation.


StressMark antibodies supplies antibodies that are for research of human proteins.

Antibody's recommended dilutions for use

WB (1:2000), IHC (1:200), ICC/IF (1:500); optimal dilutions for assays should be determined by the user.

Antibody's other name

hMAD-2 Antibody, JV18 Antibody, MAD Antibody, MAD homolog 2 Antibody, SMAD 2 Antibody, SMAD family member 2 Antibody

Primary research fields

Cell Signaling, Epigenetics and Nuclear Signalling, Nuclear Signaling Pathways, SMADs, Stem Cells, Signaling Pathways, TGF Beta, Cytoplasmic


This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Certificate of analysis

A 1:1000 dilution of SPC-1284 was sufficient for detection of Smad2 in 10 µg of Hek293 cell lysates by ECL immunoblot analysis using Goat Anti-Rabbit IgG:HRP as the secondary antibody.


Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Representative figure legend

Western blot analysis of Human Hek293 cell lysates showing detection of ~52kDa Smad2 protein using Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284). Lane 1: Human Hek293 treated with the immunizing peptide. Lane 2: Human Hek293. Primary Antibody: Rabbit Anti-Smad2 Polyclonal Antibody (SPC-1284) at 1:1000. Predicted/Observed Size: ~52kDa. Rabbit Anti-Smad2 Antibody used in Western blot (WB) on Hek293 cell lysates (SPC-1284)


1. Heldin C.H. et al. (1997) Nature. 390: 465-471. 2. Attisano L. and Wrana, J.L. (1998) Curr Opin Cell Biol. 10: 188-194. 3. Massagué J. (1998) Annu Rev Biochem. 67: 753-791. 4. Whitman M. (1998) Genes Dev. 12: 2445-2462. 5. Wu G. et al. (2000) Science. 287: 92-97. 6. Abdollah S., et al. (1997) J. Biol. Chem. 272: 27678-27685. 7. Souchelnytskyi S., et al. (1997) J. Biol. Chem. 272: 28107-28115. 8. Liu X., et al. (1997) Proc. Natl. Acad. Sci. USA. 94: 10669-10674.

Scientific context

Members of the Smad family of cell signaling molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. There are three distinct classes of Smads: the receptor-regulated Smads (R-Smads), the common-mediator Smad (co-Smad), and the antagonistic or inhibitory Smads. Following stimulation by TGF-β, Smad2 and Smad3 become phosphorylated at their carboxyl termini (Ser465 and 467 on Smad2; Ser423 and 425 on Smad3) by TGF-β Receptor I. Phosphorylated Smad 2/3 can complex with Smad4, translocate to the nucleus and regulate gene expression.


Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by StressMark antibodies. Rabbit antibodies are very stable and can be stored for several days at room temperature. StressMark antibodies adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.