Smad3 (Ser423 + Ser425) Antibody, ALEXA FLUOR 488

Basic information

Name

Smad3 (Ser423 + Ser425) Antibody, ALEXA FLUOR 488

size

100ul

Catalog number

bs-3425R-A488

Price

350 €

Details & buy    

Extended information

Gene ID Number

4088

Swiss Prot

P84022

French translation

anticorps

Tested applications

IF(IHC-P)

Clonality

Polyclonal

Conjugation

Alexa Fluor

Concentration

1ug per 1ul

Immunogen range

380-425/425

Excitation emission

499nm/519nm

Modification Site

Ser423 + Ser425

Modification

Phosphorylation

Conjugated with

ALEXA FLUOR® 488

Crossreactivity

Human, Mouse, Rat

Subcellular location

Cytoplasm, Nucleus

Clone

Polyclonal antibody

Recommended dilutions

IF(IHC-P)(1:50-200)

Target Antigen

Smad3 Ser423 + Ser425

Purification

Purified by Protein A.

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Smad3 Ser423 + Ser425 PAb ALEXA FLUOR 488

Long name

Smad3 (Ser423 + Ser425) Antibody, ALEXA FLUOR 488 Conjugated

Specificity

This is a highly specific antibody against Smad3 Ser423 + Ser425.

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423/425 [CS(p-S)V(p-S)]

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; SMAD family member 3; SMAD 3; Smad3; hSMAD3

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Smad3 Ser423 + Ser425 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.