Smad3 (Ser423) Antibody, ALEXA FLUOR 647

Basic information

Name

Smad3 (Ser423) Antibody, ALEXA FLUOR 647

size

100ul

Catalog number

bs-2225R-A647

Price

350 €

Details & buy    

Extended information

Gene ID Number

4088

Modification Site

Ser423

Tested applications

IF(IHC-P)

French translation

anticorps

Clonality

Polyclonal

Excitation emission

650nm/665nm

Concentration

1ug per 1ul

Target Antigen

Smad3 Ser423

Modification

Phosphorylation

Crossreactivity

Human, Mouse, Rat

Conjugated with

ALEXA FLUOR® 647

Recommended dilutions

IF(IHC-P)(1:50-200)

Clone

Polyclonal antibody

Purification

Purified by Protein A.

Conjugation

Alexa Fluor,ALEXA FLUOR 647

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Also known as

Anti-Smad3 Ser423 PAb ALEXA FLUOR 647

Specificity

This is a highly specific antibody against Smad3 Ser423.

Long name

Smad3 (Ser423) Polyclonal Antibody, ALEXA FLUOR 647 Conjugated

Cross-reactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Source

KLH conjugated synthetic phosphopeptide derived from human Smad3 around the phosphorylation site of Ser423

Storage conditions

Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.

Synonyms

Smad3 phospho Ser423; Smad3 phospho S423; p-Smad3 Ser423; hMAD 3; hSMAD3; HSPC193; JV15 2; JV152; MAD mothers against decapentaplegic Drosophila homolog 3; MAD3; MADH 3; MADH3; Mothers against decapentaplegic homolog 3; Mothers against DPP homolog 3; SMA and MAD related protein 3; SMAD 3; SMAD; SMAD-3; SMAD3_HUMAN.

Properties

For facs or microscopy Alexa 1 conjugate.Alexa Fluor 633 is a practical alternative to APC as well as Cy5. Bioss Primary Conjugated Antibodies. ALEXA FLUOR made this Alexa Fluor 633 conjugate that can be used in multi-color flow cytometry with instruments equipped with a second red laser or red diode. It is detected in the FL4 detector of the core's upgraded 2-laser FACScans. Like other Alexa Fluor dyes, the Anti-Smad3 Ser423 exhibits uncommon photo stability, making it an ideal choice for fluorescent microscopy.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Background of the antigen

Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development. The Smad family is divided into three subclasses: receptor regulated Smads, activin/TGF beta receptor regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5, and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7). Activated Smad3 oligomerizes with Smad4 upon TGF beta stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors. Phosphorylation of the two TGF beta dependent serines 423 and 425 in the C terminus of Smad3 is critical for Smad3 transcriptional activity and TGF beta signaling.