anti- SMAD2 antibody

Basic information

Name

anti- SMAD2 antibody

size

2X100μg

Catalog number

FNab07993

Price

320 €

Details & buy    

Extended information

Uniprot ID

Q15796

Calculated MW

52 kDa

Form

liquid

French translation

anticorps

Specificity

Human, Rat

Tested Application

ELISA, WB, IHC

Immunogen

SMAD family member 2

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Recommended dilution

WB: 1:500 - 1:2000; IHC: 1:50 - 1:100

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3 , -20℃ for 24 months (Avoid repeated freeze / thaw cycles.)

Image4

HeLa cells were subjected to SDS PAGE followed by western blot with FNab07993(SMAD2 antibody) at dilution of 1:1000

Image1

Immunohistochemistry of paraffin-embedded human endometrial cancer tissue slide using FNab07993( SMAD2 Antibody) at dilution of 1:50

Properties

If you buy Antibodies supplied by FineTest they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Synonyms

MADH2, MADR2, hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody for research use.

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants have been observed for this gene.