MADH7 Antibody

Basic information

Name

MADH7 Antibody

size

0,1 mg

Catalog number

PA1954

Price

320 €

Extended information

Target antigen

MADH7

Reacts with species:

human

Gene Name

SMAD7

Raised in

rabbit

Uniprot ID

O15105

Analyses

WB,IHC-P

French translation

anticorps

Product form

freeze-dried

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Gene Full Name

SMAD family member 7

Type of the antibody

IgG polyclonal antibody

Purification

Immunogen affinity purified.

Protein Name

Mothers against decapentaplegic homolog 7

Product configuration

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Solubilization

The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human MADH7(375-389aa YSLQRPNDHEFMQQP), identical to the related rat and mouse sequences.

Tips

The MADH7 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Storage condtions

Keep the MADH7 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Related articles

1. Lallemand, F., Mazars, A., Prunier, C., Bertrand, F., Kornprost, M., Gallea, S., Roman-Roman, S., Cherqui, G., Atfi, A. Smad7 inhibits the survival nuclear factor kappa-B and potentiates apoptosis in epithelial cells. Oncogene 20: 879-884, 2001. 2. Roijer, E., Moren, A., ten Dijke, P., Stenman, G. Assignment of the Smad7 gene (MADH7) to human chromosome 18q21.1 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 81: 189-190, 1998. 3. Topper, J. N., Cai, J., Qiu, Y., Anderson, K. R., Xu, Y.-Y., Deeds, J. D., Feeley, R., Gimeno, C. J., Woolf, E. A., Tayber, O., Mays, G. G., Sampson, B. A., Schoen, F. J., Gimbrone, M. A., Jr., Falb, D. Vascular MADs: two novel MAD-related genes selectively inducible by flow in human vascular endothelium. Proc. Nat. Acad. Sci. 94: 9314-9319, 1997.

Synonyms

FLJ16482 antibody|hSMAD 7 antibody|hSMAD7 antibody|MAD(mothers against decapentaplegic Drosophila) homolog 7 antibody|MAD antibody|Mad homolog 7 antibody|MAD homolog 8 antibody|MAD mothers against decapentaplegic homolog 7 antibody|MADH 7 antibody|MADH 8 antibody|MADH6 antibody|MADH8 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody|Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody|Mothers against decapentaplegic homolog 7 antibody|Mothers against decapentaplegic homolog 8 antibody|Mothers against DPP homolog 7 antibody|Mothers against DPP homolog 8 antibody|SMA- AND MAD-RELATED PROTEIN 7 antibody|SMAD 7 antibody|SMAD antibody|SMAD family member 7 antibody|SMAD, mothers against DPP homolog 7(Drosophila) antibody|SMAD, mothers against DPP homolog 7 antibody|SMAD6 antibody|SMAD7 antibody|SMAD7_HUMAN antibody

Background

SMAD7(Mother against decapentaplegic drosophila homolog of 7), also known as MADH7 or SMA- AND MAD-RELATED PROTEIN 7, is a protein that in humans is encoded by the SMAD7 gene. It belongs to the SMAD family of proteins, which belong to the TGFbeta superfamily of ligands. By somatic cell hybrid analysis, Topper et al.(1997) mapped the MADH7 gene to human chromosome 18. Topper et al.(1997) demonstrated that MADH7 and MADH6 could form complexes in endothelial cells. MADH7 was induced in cultured vascular endothelium by fluid mechanical forces and was capable of modulating endothelial gene expression in response to both humoral and biomechanical stimuli in vitro. By FISH, Roijer et al.(1998) refined the localization to 18q21.1. Lallemand et al.(2001) showed that cells stably expressing SMAD7 had increased susceptibility to apoptosis induced by TGFB, TNFA, serum withdrawal, or loss of cell adhesion.