SMAD 1/2/3/5 Antibody

Basic information

Name

SMAD 1/2/3/5 Antibody

size

0,1 mg

Catalog number

PA1331

Price

320 €

Extended information

Analyses

WB

Raised in

rabbit

Uniprot ID

Q15797

French translation

anticorps

Product form

freeze-dried

Target antigen

SMAD 1/2/3/5

Clone

Polyclonal antibody

Clonality

Polyclonal antibody

Gene Name

SMAD1|SMAD2|SMAD3|SMAD5

Type of the antibody

IgG polyclonal antibody

Gene Full Name

SMAD family member 1/2/3/5

Purification

Immunogen affinity purified.

Protein Name

Mothers against decapentaplegic homolog 1/2/3/5

Reacts with species:

Human, Mouse, Rat. No cross reactivity with other proteins.

Product configuration

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Synonyms

BSP 1 | BSP1 | BSP-1 | BSP1, MADH1, MADR1 | HsMAD1 | JV4 1 | JV4-1 | MADH1 | Madh1, Madr1 | Q15797 | SMAD1

Solubilization

The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human SMAD 1/2/3/5(442-456aa GPLQWLDKVLTQMGS), identical to the related mouse and rat sequences.

Related articles

1. Zhu, H., Kavsak, P., Abdollah, S., Wrana, J. L., Thomsen, G. H. A SMAD ubiquitin ligase targets the BMP pathway and affects embryonic pattern formation. Nature 400: 687-693, 1999.

Tips

The SMAD 1/2/3/5 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Storage condtions

Keep the SMAD 1/2/3/5 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Background

SMADs are proteins that modulate the activity of transforming growth factor beta ligands. The SMADs, often in complex with other SMADs/CoSMAD, act as transcription factors that regulate the expression of certain genes. Zhu, H et al concluded that targeted ubiquitination of SMADs may serve to control both embryonic development and a wide variety of cellular responses to TGF-beta signals. R-Smads or receptor regulated Smads are a class of proteins that include SMAD1, SMAD2, SMAD3, SMAD5, and SMAD8. In response to signals by the TGF-beta superfamily of ligands these proteins associate with receptor kinases and are phosphorylated at an SSXS motif at their extreme C-terminus. These proteins then typically bind to the common mediator Smad or co-SMAD SMAD4.