SMAD2/3 Antibody

Basic information

Name

SMAD2/3 Antibody

size

0,1 mg

Catalog number

PA1073-1

Price

320 €

Details & buy    

Extended information

Raised in

rabbit

Target antigen

SMAD2/3

Gene Name

SMAD2/3

Product form

solution

French translation

anticorps

Uniprot ID

Q15796/P84022

Analyses

WB,IHC-P,IHC-F

Clonality

Polyclonal antibody

Clone

Polyclonal antibody

Gene Full Name

SMAD family member 2/3

Type of the antibody

IgG polyclonal antibody

Purification

Immunogen affinity purified.

Reacts with species:

human, rat Predicted to work with: mouse

Protein Name

Mothers against decapentaplegic homolog 2/3

Product configuration

Each vial contains 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4.

Solubilization

The powdered antibody should be dissolved in 0.2 ml of distilled water to achieve final concentration of 500ug/ml

Properties

If you buy Antibodies supplied by boster they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SMAD2/3(32-47aa EQNGQEEKWCEKAVKS), identical to the related mouse and rat sequences.

Tips

The SMAD2/3 Antibody did not cross-reacted with other proteins during the test procedures. This antobdy is intended to be used for research analyses and it is not applicale for in vitro diagnostics.

Storage condtions

Keep the SMAD2/3 Antibodyat minus twenty degrees Celsius for 1 year. The ready-to-use solutions can be stored at four degrees Celsius for a month. Our specialsits recommend to freeze the aliquotes at minus twenty degrees Celsius for long-term application. Multiple procedures of freezing and thawing influence the specifity and reactivity of the antibody in a negative way.

Background

SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Transforming growth factor(TGF)-beta stimulation leads to phosphorylation and activation of Smad2 and Smad3, which form complexes with Smad4 that accumulate in the nucleus and regulate transcription of target genes. Smad2 and Smad3 share highly homology. SMAD2/SMAD3 signal transduction appeared to be important in the regulation of muscle-specific genes. SMAD proteins transmit signals from transmembrane serine/threonine kinase receptors to the nucleus. Smad2 is a 58 kDa member of a family of proteins involved in cell proliferation, differentiation and development.Smad3 is a 50 kDa member of a family of proteins that act as key mediators of TGF beta superfamily signaling in cell proliferation, differentiation and development.